This web page was created as an assignment for Genetics 677, an undergraduate course at UW-Madison.
Protein Motifs and Domains
Motif Analysis
LMNA codes for type V lamins which include nuclear lamins. All lamins are evolutionary and structurally similar. Each serve as scaffolding in the cell, nuclear lamins providing structural support for the nuclear membrane. Since they are all intermediate filaments it is expected that the only motifs found were intermediate filaments and IF tail domain. These were the only motifs found using Pfam and Prosite. I did not use any other motif finders since it would not be expected to see any other motifs. The highlighted sequence to the left was the motif found on Prosite for intermediate filaments.
Domain Analysis
The motifs found were for IFs and the IF tail domain. IF proteins share a common structural composition consisting of ~350 nt coiled-coiled alpha helices rod domain. Also included is a C-terminus head domain and an N-terminus tail domain. The tail domain is important since it is used to characterize the lamin, whether it is type I-V. Important to note that the exact structure of the IF tail domain of lamin A is not yet characterized and several structures seem viable. (Goldman 2009)
Intemediate Filament IF Tail Domain
References
Quinlan R, Hutchison C, Lane B; , Protein Profile 1995;2:801-951.: Intermediate filament proteins. PUBMED:8771189
Prosite info: ScanProsite
Robert Goldman. Distinguished Lecture Series on Nuclear Lamins.
Peter St. Andre [email protected] Last updated:2/3/09
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